Overview of Ligand Binding Module
Features in Ligand Binding Module
Report Generation in Ligand Binding Module
Graphs in Ligand Binding Module
Ligand Binding Module Equations
Ligard Binding Module Requirement
Product Uses
Support
The Enzyme Kinetics Module is a SigmaPlot add-on that guides you through data entry, analysis and graphing of your enzyme kinetics data. Select the study type in the customized Data Entry Wizard, choose from 56 built-in equations, then discover the best fit to characterize the reaction mechanism. Automatically display a series of graphs to quickly determine the type of inhibition. The module continues SigmaPlot's tradition of award-winning interface, ease-of-use and intelligent wizards to guide you through your analysis.
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Automatically fit your radioligand and dose response studies for multiple compounds with replicate data. Choose from over 10 built-in equations, create a new equation or modify and existing equation to gain insight on the number of binding sites, and their affinity and accessibility for various drugs. Automatically generate a plot of mean response versus concentration and include the best-fit lines for each compound.
OVERVIEW of SIGMAPLOT LIGAND BINDING MODULES


TOPICS


Analyze and plot ligand/receptor and dose response data quickly and easily

Automatically fit radioligand and dose response equations for multiple compounds with replicate data. Select the type of study in the customized dialog and specify the number of replicate values to instantly plot and analyze your data. All graphs and analysis appear as notebook items, to efficiently manage your work.

Select from a wide range of models to determine parameter values

Choose from ten built-in equations, create a new equation or modify an existing equation to analyze the number of binding sites, and their affinity for various drugs. Fit one and two site saturation equations to determine the number of binding sites, Bmax, and the ligand affinity, Kd. Fit competition and sigmoidal dose-response equations to learn the affinity of the receptor for the competing molecule, EC50, or specify ligand concentration and Kd to generate Ki, the equilibrium dissociation constant Automatically generate a report with the equation fit results and the associated error analysis.

  

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Save time by plotting multiple compounds simultaneously

Automatically generate a plot of mean response versus concentration and include the best-fit lines for each compound. Modify your graph as desired to present your results exactly the way you want. Specify the fit range, minimum and maximum x values, to customize your analysis. Clearly distinguish different compounds with symbols and legends.

The Ligand Binding Module is included FREE on the SigmaPlot 8.0 CD-ROM and can be downloaded for use with SigmaPlot 7.1 or higher

 

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